2025
Bloom J, Green RA, Desai A, Oegema K, Rifkin SA. Hybrid incompatibility emerges at the one-cell stage in interspecies Caenorhabditis embryos. Curr Biol. 2025 Jul 21;35(14):3530-3541
Bellaart A, Brambila A, Xu J, Mendez Diaz F, Deep A, Anzola J, Meitinger F, Ohta M, Corbett KD, Desai A, Oegema K. (2025) TRIM37 prevents ectopic spindle pole assembly by peptide motif recognition and substrate-dependent oligomerization. Nat Struct Mol Biol. 2025 May 25.
2024
Houston J, Vissotsky C, Deep A, Hakozaki H, Crews E, Oegema K, Corbett KD, Lara-Gonzalez P, Kim T, Desai A. Phospho-KNL-1 recognition by a TPR domain targets the BUB-1-BUB-3 complex to C. elegans kinetochores. J. Cell Biol. 2024 Jul 1;223(7)
Schlientz AJ, Lee KY, Sebastián Gómez-Cavazos J, Lara-González P, Desai A, Oegema K. (2024) The CYK-4 GAP domain regulates cortical targeting of centralspindlin to promote contractile ring assembly and facilitate ring dissolution. bioRxiv [Preprint]. 2024 Oct 30:2024.
Sankaralingam P, Wang S, Liu Y, Oegema KF, O'Connell KF. (2024) The kinase ZYG-1 phosphorylates the cartwheel protein SAS-5 to drive centriole assembly in C. elegans. EMBO Rep. 2024 Jun;25(6):2698-2721.
Green RA, Khaliullin RN, Zhao Z, Ochoa SD, Hendel JM, Chow TL, Moon H, Biggs RJ, Desai A, Oegema K. (2024) Automated profiling of gene function during embryonic development. Cell. 2024 Jun 6;187(12):3141-3160.
Meitinger F, Belal H, Davis RL, Martinez MB, Shiau AK, Oegema K, Desai A. (2024) Control of cell proliferation by memories of mitosis. Science. 2024 Mar 29;383(6690)
Lara-Gonzalez P, Variyar S, Moghareh S, Nguyen ACN, Kizhedathu A, Budrewicz J, Schlientz A, Varshney N, Bellaart A, Oegema K, Bardwell L, Desai A (2024) Cyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses. J Cell Biol. 2024 Nov 4;223(11)
2023
Bai X, Green R, Cai T, Oegema K, Golden A.(2023) A missense mutation in the C. elegans src-2 tyrosine-protein kinase reduces brood size and enhances embryonic morphogenesis defects in src-1(RNAi) conditions. MicroPubl Biol. 2023 Jul 11;2023
Houston J, Ohta M, Gómez-Cavazos JS, Deep A, Corbett KD, Oegema K, Lara-Gonzalez P, Kim T, Desai A. (2023) BUB-1-bound PLK-1 directs CDC-20 kinetochore recruitment to ensure timely embryonic mitoses. Curr Biol. 2023 Jun 5;33(11):2291-2299.e10.
2022
Hattersley N, Schlientz AJ, Prevo B, Oegema K, Desai A. (2022) MEL-28/ELYS and CENP-C coordinately control outer kinetochore assembly and meiotic chromosome-microtubule interactions. Curr Biol. 2022 Jun 6;32(11):2563-2571.
2021
Meitinger F, Kong D, Ohta M, Desai A, Oegema K, Loncarek J. (2021) TRIM37 prevents formation of condensate-organized ectopic spindle poles to ensure mitotic fidelity. J Cell Biol. 2021 Jul 5;220(7):e202010180.
Kawashima AT, Wong C, Lordén G, King CC, Lara-Gonzalez P, Desai A, Gingras AC, Newton AC. (2021) The PHLPP1 N-Terminal Extension Is a Mitotic Cdk1 Substrate and Controls an Interactome Switch. Molecular and Cellular Biology. 41(3).
de Groot C, Houston J, Davis B, Gerson-Gurwitz A, Monen J, Lara-Gonzalez P, Oegema K, Shiau AK, Desai A. (2021) The N-terminal tail of C. elegans CENP-A interacts with KNL-2 and is essential for centromeric chromatin assembly. Mol Biol Cell. 2021 Jun 1;32(12)
Ohta M, Zhao Z, Wu D, Wang S, Harrison JL, Gómez-Cavazos JS, Desai A, Oegema KF. (2021) Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosome. Journal of Cell Biology. 220(2).
Lara-Gonzalez P, Kim T, Oegema K, Corbett K, Desai A. (2021) A tripartite mechanism catalyzes Mad2-Cdc20 assembly at unattached kinetochores. Science. 2021 Jan 1;371(6524):64-7.
2020
Meitinger F, Ohta M, Lee KY, Watanabe S, Davis RL, Anzola JV, Kabeche R, Jenkins DA, Shiau AK, Desai A, Oegema K. (2020) TRIM37 controls cancer-specific vulnerability to PLK4 inhibition. Nature. 1-7
Gómez-Cavazos JS, Lee KY, Lara-González P, Li Y, Desai A, Shiau AK, Oegema K. (2020) A non-canonical BRCT-phosphopeptide recognition mechanism underlies RhoA activation in cytokinesis. Curr Biol. 30(16):3101-15
Houston J, Lara-Gonzalez P, Desai A. (2020) Rashomon at the kinetochore: Function(s) of the Mad1–cyclin B1 complex. J Cell Biol. 219(8)
Watanabe S, Meitinger F, Shiau AK, Oegema K, Desai A. (2020) Centriole-independent mitotic spindle assembly relies on the PCNT–CDK5RAP2 pericentriolar matrix. Journal of Cell Biology. 219(12).
2019
Lara-Gonzalez P, Moyle MW, Budrewicz J, Mendoza-Lopez J, Oegema K, Desai A. (2019) The G2-to-M transition is ensured by a dual mechanism that protects cyclin B from degradation by Cdc20-activated APC/C. Dev Cell. 51(3):313-25
Gerson-Gurwitz A, Worby CA, Lee KY, Khaliullin R, Bouffard J, Cheerambathur D, Oegema K, Cram EJ, Dixon JE, Desai A. (2019) Ancestral roles of the Fam20C family of secreted protein kinases revealed in C. elegans. J Cell Biol. 218(11):3795-811
Khaliullin RN, Hendel JM, Gerson-Gurwitz A, Wang S, Ochoa SD, Zhao Z, Desai A, Oegema K, Green RA. (2019) A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development. J Vis Exp. (152):e60362
Gemble S, Simon A, Pennetier C, Dumont M, Hervé S, Meitinger F, Oegema K, Rodriguez R, Almouzni G, Fachinetti D, Basto R. (2019) Centromere Dysfunction Compromises Mitotic Spindle Pole Integrity. Curr Biol. 29(18):3072-80
Dabydeen SA, Desai A, Sahoo D. (2019) Unbiased Boolean analysis of public gene expression data for cell cycle gene identification. Mol Biol Cell. 30(14):1770-9
Chen AL, Lum KM, Lara-Gonzalez P, Ogasawara D, Cognetta AB, To A, Parsons WH, Simon GM, Desai A, Petrascheck M, Bar-Peled L. (2019) Pharmacological convergence reveals a lipid pathway that regulates C. elegans lifespan. Nat Chem Biol. 15(5):453-62
Wang S, Ochoa SD, Khaliullin RN, Gerson-Gurwitz A, Hendel JM, Zhao Z, Biggs R, Chisholm AD, Desai A, Oegema K, Green RA. (2019) A high-content imaging approach to profile C. elegans embryonic development. Development. 146(7)
Cheerambathur DK, Prevo B, Chow TL, Hattersley N, Wang S, Zhao Z, Kim T, Gerson-Gurwitz A, Oegema K, Green R, Desai A. (2019) The kinetochore-microtubule coupling machinery is repurposed in sensory nervous system morphogenesis. Dev Cell. 48(6):864-72
Chan FY, Silva AM, Saramago J, Pereira-Sousa J, Brighton HE, Pereira M, Oegema K, Gassmann R, Carvalho AX. (2019) The ARP2/3 complex prevents excessive formin activity during cytokinesis. Mol Biol Cell. 30(1):96-107
2018
Oegema K, Davis RL, Lara-Gonzalez P, Desai A, Shiau AK. (2018) CFI-400945 is not a selective cellular PLK4 inhibitor. Proc Natl Acad Sci USA. 115(46):E10808-e10809
Desai A, Jun S. (2018) Promoting an "Auteur Theory" for Young Scientists: Preserving Excitement and Creativity. Bioessays. 40(11):e1800147
Lee KY, Green RA, Gutierrez E, Gomez-Cavazos JS, Kolotuev I, Wang S, Desai A, Groisman A, Oegema K. (2018) CYK-4 functions independently of its centralspindlin partner ZEN-4 to cellularize oocytes in germline syncytia. Elife. 7: e36919
Khaliullin RN, Green RA, Shi LZ, Gomez-Cavazos JS, Berns MW, Desai A, Oegema K. (2018) A positive-feedback-based mechanism for constriction rate acceleration during cytokinesis in Caenorhabditis elegans. Elife. 7:e36073
Hattersley N, Lara-Gonzalez P, Cheerambathur D, Gomez-Cavazos JS, Kim T, Prevo B, Khaliullin R, Lee KY, Ohta M, Green R, Oegema K, Desai A. (2018) Employing the one-cell C. elegans embryo to study cell division processes. Methods Cell Biol. 144185-231
Quintin S, Wang S, Pontabry J, Bender A, Robin F, Hyenne V, Landmann F, Gally C, Oegema K, Labouesse M. (2018) Correction: Non-centrosomal epidermal microtubules act in parallel to LET-502/ROCK to promote C. elegans elongation (doi:10.1242/dev.126615). Development. 145(10)
Nene RV, Putnam CD, Li BZ, Nguyen KG, Srivatsan A, Campbell CS, Desai A, Kolodner RD. (2018) Cdc73 suppresses genome instability by mediating telomere homeostasis. PLoS Genet. 14(1):e1007170
Mangal S, Sacher J, Kim T, Osorio DS, Motegi F, Carvalho AX, Oegema K, Zanin E. (2018) TPXL-1 activates Aurora A to clear contractile ring components from the polar cortex during cytokinesis. J Cell Biol. 217(3):837-848
2017
Kim T, Desai A. (2017) Meiosis: The Origins of Bias. Curr Biol. 27(24):R1309-r1311
Lara-Gonzalez P, Kim T, Desai A. (2017) Taming the Beast: Control of APC/C(Cdc20)-Dependent Destruction. Cold Spring Harb Symp Quant Biol. 82:111-121
Martino L, Morchoisne-Bolhy S, Cheerambathur DK, Van Hove L, Dumont J, Joly N, Desai A, Doye V, Pintard L. (2017) Channel Nucleoporins Recruit PLK-1 to Nuclear Pore Complexes to Direct Nuclear Envelope Breakdown in C. elegans. Dev Cell. 43(2):157-171.e157
Kim T, Lara-Gonzalez P, Prevo B, Meitinger F, Cheerambathur DK, Oegema K, Desai A. (2017) Kinetochores accelerate or delay APC/C activation by directing Cdc20 to opposing fates. Genes Dev. 31(11):1089-1094
Santaguida S, Richardson A, Iyer DR, M'Saad O, Zasadil L, Knouse KA, Wong YL, Rhind N, Desai A, Amon A. (2017) Chromosome Mis-segregation Generates Cell-Cycle-Arrested Cells with Complex Karyotypes that Are Eliminated by the Immune System. Dev Cell. 41(6):638-651.e635
Wang S, Tang NH, Lara-Gonzalez P, Zhao Z, Cheerambathur DK, Prevo B, Chisholm AD, Desai A, Oegema K. (2017) A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans. Development. 144(14):2694-2701
Cheerambathur DK, Prevo B, Hattersley N, Lewellyn L, Corbett KD, Oegema K, Desai A. (2017) Dephosphorylation of the Ndc80 Tail Stabilizes Kinetochore-Microtubule Attachments via the Ska Complex. Dev Cell. 41(4):424-437.e424
Fink S, Turnbull K, Desai A, Campbell CS. (2017) An engineered minimal chromosomal passenger complex reveals a role for INCENP/Sli15 spindle association in chromosome biorientation. J Cell Biol. 216(4):911-923
Coller HA, Desai A. (2017) Cell cycle, cell division, and cell death. Mol Biol Cell. 28(6):693-694
Ohta M, Desai A, Oegema K. (2017) How centrioles acquire the ability to reproduce. Elife. 6:e25358
Musacchio A, Desai A. (2017) A Molecular View of Kinetochore Assembly and Function. Biology (Basel). 6(1):
Hattersley N, Desai A. (2017) The nucleoporin MEL-28/ELYS: A PP1 scaffold during M-phase exit. Cell Cycle. 16(6):489-490
2016
Xing M, Peterman MC, Davis RL, Oegema K, Shiau AK, Field SJ. (2016) GOLPH3 drives cell migration by promoting Golgi reorientation and directional trafficking to the leading edge. Mol Biol Cell. 27(24):3828-3840
Hattersley N, Cheerambathur D, Moyle M, Stefanutti M, Richardson A, Lee KY, Dumont J, Oegema K, Desai A. (2016) A Nucleoporin Docks Protein Phosphatase 1 to Direct Meiotic Chromosome Segregation and Nuclear Assembly. Dev Cell. 38(5):463-477
Wueseke O, Zwicker D, Schwager A, Wong YL, Oegema K, Jülicher F, Hyman AA, Woodruff JB. (2016) Polo-like kinase phosphorylation determines Caenorhabditis elegans centrosome size and density by biasing SPD-5 toward an assembly-competent conformation. Biol Open. 5(10):1431-1440
Meitinger F, Anzola JV, Kaulich M, Richardson A, Stender JD, Benner C, Glass CK, Dowdy SF, Desai A, Shiau AK, Oegema K. (2016) 53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration. J Cell Biol. 214(2):155-166
Cheerambathur DK, Prevo B, Desai A. (2016) A TOGgle for Tension at Kinetochores. Cell. 165(6):1316-1318
Gerson-Gurwitz A, Wang S, Sathe S, Green R, Yeo GW, Oegema K, Desai A. (2016) A Small RNA-Catalytic Argonaute Pathway Tunes Germline Transcript Levels to Ensure Embryonic Divisions. Cell. 165(2):396-409
Kim T, Moyle MW, Lara-Gonzalez P, De Groot C, Oegema K, Desai A. (2016) Correction: Kinetochore-localized BUB-1/BUB-3 complex promotes anaphase onset in C. elegans. J Cell Biol. 212(6):737
Quintin S, Wang S, Pontabry J, Bender A, Robin F, Hyenne V, Landmann F, Gally C, Oegema K, Labouesse M. (2016) Non-centrosomal epidermal microtubules act in parallel to LET-502/ROCK to promote C. elegans elongation. Development. 143(1):160-173
2015
de Groot CO, Hsia JE, Anzola JV, Motamedi A, Yoon M, Wong YL, Jenkins D, Lee HJ, Martinez MB, Davis RL, Gahman TC, Desai A, Shiau AK. (2015) A Cell Biologist's Field Guide to Aurora Kinase Inhibitors. Front Oncol. 5285
Basto R, Oegema K. (2015) Methods in Cell Biology. Centrosome & Centriole. Preface. Methods Cell Biol. 129xvii-xix
Wang S, Wu D, Quintin S, Green RA, Cheerambathur DK, Ochoa SD, Desai A, Oegema K. (2015) NOCA-1 Functions with γ-tubulin and in parallel to Patronin to assemble non-centrosomal microtubule arrays in C. elegans. Elife. 4e08649
Espeut J, Lara-Gonzalez P, Sassine M, Shiau AK, Desai A, Abrieu A. (2015) Natural loss of Mps1 kinase in nematodes uncovers a role for Polo-like kinase 1 in spindle checkpoint initiation. Cell Rep. 12(1):58-65
Shen Y, Li J, Nitta M, Futalan D, Steed T, Treiber JM, Taich Z, Stevens D, Wykosky J, Chen HZ, Carter BS, Becher OJ, Kennedy R, Esashi F, Sarkaria JN, Furnari FB, Cavenee WK, Desai A, Chen CC. (2015) Orthogonal targeting of EGFRvIII expressing glioblastomas through simultaneous EGFR and PLK1 inhibition. Oncotarget. 6(14):11751-11767
Kim T, Moyle MW, Lara-Gonzalez P, De Groot C, Oegema K, Desai A. (2015) Kinetochore-localized BUB-1/BUB-3 complex promotes anaphase onset in C. elegans. J Cell Biol. 209(4):507-517
Woodruff JB, Wueseke O, Viscardi V, Mahamid J, Ochoa SD, Bunkenborg J, Widlund PO, Pozniakovsky A, Zanin E, Bahmanyar S, Zinke A, Hong SH, Decker M, Baumeister W, Andersen JS, Oegema K, Hyman AA. (2015) Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro. Science. 348(6236):808-812
Wong YL, Anzola JV, Davis RL, Yoon M, Motamedi A, Kroll A, Seo CP, Hsia JE, Kim SK, Mitchell JW, Mitchell BJ, Desai A, Gahman TC, Shiau AK, Oegema K. (2015) Reversible centriole depletion with an inhibitor of Polo-like kinase 4. Science. 348(6239):1155-1160
Monen J, Hattersley N, Stevens D, Essex A, Oegema K, Desai A. (2015) Separase cleaves the N-Tail of the CENP-A related protein CPAR-1 at the meiosis I metaphase-anaphase transition in C. elegans. PLoS One. 10(4):e0125382
Holland AJ, Reis RM, Niessen S, Pereira C, Andres DA, Spielmann HP, Cleveland DW, Desai A, Gassmann R. (2015) Preventing farnesylation of the dynein adaptor Spindly contributes to the mitotic defects caused by farnesyltransferase inhibitors. Mol Biol Cell. 26(10):1845-1856
Folco HD, Campbell CS, May K, Espinoza C, Oegema K, Ren B, Hardwick K, Grewal S, Desai A. (2015) The N-terminal tail of CENP-A confers epigenetic stability to centromeres via the CENP-T branch of the CCAN in fission yeast. Curr Biol. 25(3):348-56
Kern D, Kim T, Rigney M, Hattersley N, Desai A, Cheeseman IM. (2015) The outer kinetochore protein KNL-1 forms a defined oligomer in nematodes. Mol Biol Cell. 26(2):229-237
2014
Chuang M, Goncharov A, Wang S, Oegema K, Jin Y, Chisholm AD. (2014) The microtubule minus-end-binding protein patronin/PTRN-1 is required for axon regeneration in C. elegans. Cell Rep. 9(3):874-883
Corbett KD, Desai A. (2014) A new piece in the kinetochore jigsaw puzzle. J Cell Biol. 206(4):457-459
Wueseke O, Bunkenborg J, Hein MY, Zinke A, Viscardi V, Woodruff JB, Oegema K, Mann M, Andersen JS, Hyman AA. (2014) The Caenorhabditis elegans pericentriolar material components SPD-2 and SPD-5 are monomeric in the cytoplasm before incorporation into the PCM matrix. Mol Biol Cell. 25(19):2984-2992
Goellner EM, Smith CE, Campbell CS, Hombauer H, Desai A, Putnam CD, Kolodner RD. (2014) PCNA and Msh2-Msh6 activate an Mlh1-Pms1 endonuclease pathway required for Exo1-independent mismatch repair. Mol Cell. 55(2):291-304.
Shimanovskaya E, Viscardi V, Lesigang J, Lettman MM, Qiao R, Svergun DI, Round A, Oegema K, Dong G. (2014) Structure of the C. elegans ZYG-1 cryptic polo box suggests a conserved mechanism for centriolar docking of Plk4 kinases. Structure. 22(8):1090-1104
Campbell CS, Hombauer H, Srivatsan A, Bowen N, Gries K, Desai A, Putnam CD, Kolodner RD. (2014) Mlh2 Is an accessory factor for DNA mismatch repair in Saccharomyces cerevisiae. PLoS Genet. 10(5):e1004327
Moyle M, Kim T, Hattersley N, Espeut J, Cheerambathur D, Oegema D, and Desai A. (2014) A Bub1–Mad1 interaction targets the Mad1–Mad2 complex to unattached kinetochores to initiate the spindle checkpoint. J Cell Biol. 204(5):647-657
Cheerambathur D, Desai A. (2014) Linked In: formation and regulation of microtubule attachments during chromosome segregation. Curr Opin Cell Biol. 26C:113-122.
Bahmanyar S, Biggs R, Schuh AL, Desai A, Müller-Reichert T, Audhya A, Dixon JE, Oegema K. (2014) Spatial control of phospholipid flux restricts endoplasmic reticulum sheet formation to allow nuclear envelope breakdown. Genes Dev. 28(2):121-126
2013
Stevens D, Oegema K, Desai A. (2013) Meiotic Double-Strand Breaks Uncover and Protect against Mitotic Errors in the C. elegans Germline. Curr Biol. 23(23):2400-2406
Cheerambathur DK, Gassmann R, Cook B, Oegema K, Desai A. (2013) Crosstalk between microtubule attachment complexes ensures accurate chromosome segregation. Science 342(6163):1239-1242
Green RA, Mayers JR, Wang S, Lewellyn L, Desai A, Audhya A, Oegema K. (2013) The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules. J Cell Biol. 203(3):505-520
Smith CE, Mendillo ML, Bowen N, Hombauer H, Campbell CS, Desai A, Putnam CD, Kolodner RD. (2013) Dominant mutations in S. cerevisiae PMS1 identify the Mlh1-Pms1 endonuclease active site and an exonuclease 1-independent mismatch repair pathway. PLOS Genet. 9(10):e1003869
Cottee MA, Muschalik N, Wong YL, Johnson CM, Johnson S, Andreeva A, Oegema K, Lea SM, Raff JW, van Breugel M. (2013) Crystal structures of the CPAP/STIL complex reveal its role in centriole assembly and human microcephaly. Elife. 2:e01071
Zanin E, Desai A, Poser I, Toyoda Y, Andree C, Moebius C, Bickle M, Conradt B, Piekny A, Oegema K. (2013) A conserved RhoGAP limits M phase contractility and coordinates with microtubule asters to confine RhoA during cytokinesis. Dev Cell. 26(5):496-510
Varma D, Wan X, Cheerambathur D, Gassmann R, Suzuki A, Lawrimore J, Desai A, Salmon ED. (2013) Spindle assembly checkpoint proteins are positioned close to core microtubule attachment sites at kinetochores. J Cell Biol. 202(5):735-746
Fachinetti D, Diego Folco H, Nechemia-Arbely Y, Valente LP, Nguyen K, Wong AJ, Zhu Q, Holland AJ, Desai A, Jansen LE, Cleveland DW. (2013) A two-step mechanism for epigenetic specification of centromere identity and function. Nat Cell Biol. 15(9):1056-1066
Lettman MM, Wong YL, Viscardi V, Niessen S, Chen SH, Shiau AK, Zhou H, Desai A, Oegema K. (2013) Direct binding of SAS-6 to ZYG-1 recruits SAS-6 to the mother centriole for cartwheel assembly. Dev Cell. 25(3):284-298
Campbell CS, Desai A. (2013) Tension sensing by Aurora B kinase is independent of survivin-based centromere localization. Nature. 497(7447):118-21
Campbell CS, Desai A. (2013) What the hec is up with mouse oocyte meiosis? Dev Cell 25(1):3-4
2012
Olson SK, Greenan G, Desai A, Müller-Reichert T, Oegema K. (2012) Hierarchical assembly of the eggshell and permeability barrier in C. elegans. J Cell Biol. 198(4):731-748
Green RA, Paluch E, Oegema K. (2012) Cytokinesis in animal cells. Annu Rev Cell Dev Biol. 28:29-58
Gassmann R, Rechtsteiner A, Yuen KW, Muroyama A, Egelhofer T, Gaydos L, Barron F, Maddox P, Essex A, Monen J, Ercan S, Lieb JD, Oegema K, Strome S, Desai A. (2012) An inverse relationship to germline transcription defines centromeric chromatin in C. elegans. Nature. 484(7395):534-537
Dumont J, Desai A. (2012) Acentrosomal spindle assembly and chromosome segregation during oocyte meiosis. Trends Cell Biol. 22(5):241-249
Espeut J, Cheerambathur DK, Krenning L, Oegema K, Desai A. (2012) Microtubule binding by KNL-1 contributes to spindle checkpoint silencing at the kinetochore. J Cell Biol. 196(4):469-482
Maddox PS, Corbett KD, Desai A. (2012) Structure, assembly and reading of centromeric chromatin. Curr Opin Genet Dev. 22(2):139-147
Han S, Bahmanyar S, Zhang P, Grishin N, Oegema K, Crooke R, Graham M, Reue K, Dixon JE, Goodman JM. (2012) Nuclear envelope phosphatase 1-regulatory subunit 1 (formerly TMEM188) is the metazoan Spo7p ortholog and functions in the lipin activation pathway. J Biol Chem. 287(5):3123-3137
2011
Hombauer H, Campbell CS, Smith CE, Desai A, Kolodner RD. (2011) Visualization of eukaryotic DNA mismatch repair reveals distinct recognition and repair intermediates. Cell 147(5):1040-1053
Zanin E, Dumont J, Gassmann R, Cheeseman I, Maddox P, Bahmanyar S, Carvalho A, Niessen S, Yates JR 3rd, Oegema K, Desai A. (2011) Affinity purification of protein complexes in C. elegans. Methods Cell Biol. 106:289-322
Liu T, Rechtsteiner A, Egelhofer TA, Vielle A, Latorre I, Cheung MS, Ercan S, Ikegami K, Jensen M, Kolasinska-Zwierz P, Rosenbaum H, Shin H, Taing S, Takasaki T, Iniguez AL, Desai A, Dernburg AF, Kimura H, Lieb JD, Ahringer J, Strome S, Liu XS. (2011) Broad chromosomal domains of histone modification patterns in C. elegans. Genome Res. 21(2):227-236
Yuen KW, Nabeshima K, Oegema K, Desai A. (2011) Rapid De Novo Centromere Formation Occurs Independently of Heterochromatin Protein 1 in C. elegans Embryos. Curr Biol. 21(21):1800-1807
Carvalho A, Olson SK, Gutierrez E, Zhang K, Noble LB, Zanin E, Desai A, Groisman A, Oegema K.(2011) Acute drug treatment in the early C. elegans embryo. PLoS One. 6(9):e24656
Stevens D, Gassmann R, Oegema K, Desai A. (2011) Uncoordinated loss of chromatid cohesion is a common outcome of extended metaphase arrest. PLoS One. 6(8):e22969
Green RA, Kao HL, Audhya A, Arur S, Mayers JR, Fridolfsson HN, Schulman M, Schloissnig S, Niessen S, Laband K, Wang S, Starr DA, Hyman AA, Schedl T, Desai A, Piano F, Gunsalus KC, Oegema K. (2011) A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue. Cell. 145(3):470-482
Lewellyn L, Carvalho A, Desai A, Maddox AS, Oegema K. (2011) The chromosomal passenger complex and centralspindlin independently contribute to contractile ring assembly. J Cell Biol. 193(1):155-169
2010
Gerstein MB, Lu ZJ, Van Nostrand EL, Cheng C, Arshinoff BI, Liu T, Yip KY, Robilotto R, Rechtsteiner A, Ikegami K, Alves P, Chateigner A, Perry M, Morris M, Auerbach RK, Feng X, Leng J, Vielle A, Niu W, Rhrissorrakrai K, Agarwal A, Alexander RP, Barber G, Brdlik CM, Brennan J, Brouillet JJ, Carr A, Cheung MS, Clawson H, Contrino S, Dannenberg LO, Dernburg AF, Desai A, Dick L, Dose AC, Du J, Egelhofer T, Ercan S, Euskirchen G, Ewing B, Feingold EA, Gassmann R, Good PJ, Green P, Gullier F, Gutwein M, Guyer MS, Habegger L, Han T, Henikoff JG, Henz SR, Hinrichs A, Holster H, Hyman T, Iniguez AL, Janette J, Jensen M, Kato M, Kent WJ, Kephart E, Khivansara V, Khurana E, Kim JK, Kolasinska-Zwierz P, Lai EC, Latorre I, Leahey A, Lewis S, Lloyd P, Lochovsky L, Lowdon RF, Lubling Y, Lyne R, MacCoss M, Mackowiak SD, Mangone M, McKay S, Mecenas D, Merrihew G, Miller DM, 3rd, Muroyama A, Murray JI, Ooi SL, Pham H, Phippen T, Preston EA, Rajewsky N, Ratsch G, Rosenbaum H, Rozowsky J, Rutherford K, Ruzanov P, Sarov M, Sasidharan R, Sboner A, Scheid P, Segal E, Shin H, Shou C, Slack FJ, Slightam C, Smith R, Spencer WC, Stinson EO, Taing S, Takasaki T, Vafeados D, Voronina K, Wang G, Washington NL, Whittle CM, Wu B, Yan KK, Zeller G, Zha Z, Zhong M, Zhou X, Ahringer J, Strome S, Gunsalus KC, Micklem G, Liu XS, Reinke V, Kim SK, Hillier LW, Henikoff S, Piano F, Snyder M, Stein L, Lieb JD, Waterston RH. (2010) Integrative analysis of the Caenorhabditis elegans genome by the modENCODE project. Science. 330(6012):1775-1787
Folco HD, Desai A. (2010) A PSHaver for centromeric histones. Mol Cell. 40(3):351-352
Dumont J, Oegema K, Desai A. (2010) A kinetochore-independent mechanism drives anaphase chromosome separation during acentrosomal meiosis. Nat Cell Biol. 12(9):894-901
Liu W, Tanasa B, Tyurina OV, Zhou TY, Gassmann R, Liu WT, Ohgi KA, Benner C, Garcia-Bassets I, Aggarwal AK, Desai A, Dorrestein PC, Glass CK, Rosenfeld MG. (2010) PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression. Nature. 466(7305):508-512
Gassmann R, Holland AJ, Varma D, Wan X, Civril F, Cleveland DW, Oegema K, Salmon ED, Desai A. (2010) Removal of Spindly from microtubule-attached kinetochores controls spindle checkpoint silencing in human cells. Genes Dev. 24(9):957-971
Desai A, Dogterom M. (2010) Cell structure and dynamics. Curr Opin Cell Biol. 22(1):1-3.
Lewellyn L, Dumont J, Desai A, Oegema K. (2010) Analyzing the effects of delaying aster separation on furrow formation during cytokinesis in the Caenorhabditis elegans embryo. Mol Biol Cell. 21(1):50-62
2009
Dammermann A, Pemble H, Mitchell BJ, McLeod I, Yates JR 3rd, Kintner C, Desai AB, Oegema K. (2009) The hydrolethalus syndrome protein HYLS-1 links core centriole structure to cilia formation. Genes Dev. 23(17):2046-2059
Carvalho A, Desai A, Oegema K. (2009) Structural memory in the contractile ring makes the duration of cytokinesis independent of cell size. Cell 137(5):926-937
Wan X, O'Quinn RP, Pierce HL, Joglekar AP, Gall WE, DeLuca JG, Carroll CW, Liu ST, Yen TJ, McEwen BF, Stukenberg PT, Desai A, Salmon ED. (2009) Protein architecture of the human kinetochore microtubule attachment site. Cell 137(4):672-684
Essex A, Dammermann A, Lewellyn L, Oegema K, Desai A. (2009) Systematic analysis in Caenorhabditis elegans reveals that the spindle checkpoint is composed of two largely independent branches. Mol Biol Cell. 20(4):1252-1267
2008
Canman JC, Lewellyn L, Laband K, Smerdon SJ, Desai A, Bowerman B, Oegema K. (2008) Inhibition of Rac by the GAP activity of centralspindlin is essential for cytokinesis. Science. 322(5907):1543-1546
McIntosh JR, Grishchuk EL, Morphew MK, Efremov AK, Zhudenkov K, Volkov VA, Cheeseman IM, Desai A, Mastronarde DN, Ataullakhanov FI. (2008)Fibrils connect microtubule tips with kinetochores: a mechanism to couple tubulin dynamics to chromosome motion. Cell 135(2):322-333
Wilson-Kubalek EM, Cheeseman IM, Yoshioka C, Desai A, Milligan RA. (2008) Orientation and structure of the Ndc80 complex on the microtubule lattice. J Cell Biol. 182(6):1055-1061
Liu J, Desai A, Onuchic JN, Hwa T. (2008) An integrated mechanobiochemical feedback mechanism describes chromosome motility from prometaphase to anaphase in mitosis. Proc Natl Acad Sci U S A. 105(37):13752-13757
Gassmann R, Essex A, Hu JS*, Maddox PS, Motegi F, Sugimoto A, O'Rourke SM, Bowerman B, McLeod I, Yates JR 3rd, Oegema K, Cheeseman IM, Desai A. (2008) A new mechanism controlling kinetochore-microtubule interactions revealed by comparison of two dynein-targeting components: SPDL-1 and the Rod/Zwilch/Zw10 complex. Genes Dev. 22(17):2385-2399
Audhya A, Desai A. (2008) Proteomics in Caenorhabditis elegans. Brief Funct Genomic Proteomic. 7(3):205-210
Dammermann A, Maddox PS, Desai A, Oegema K. (2008) SAS-4 is recruited to a dynamic structure in newly forming centrioles that is stabilized by the gamma-tubulin-mediated addition of centriolar microtubules. J Cell Biol. 180(4):771-785
Yuen KW, Desai A. (2008) The wages of CIN. J Cell Biol. 180(4):661-663
Tanaka TU, Desai A. (2008) Kinetochore-microtubule interactions: the means to the end. Curr Opin Cell Biol. 20(1):53-63
Green RA, Audhya A, Pozniakovsky A, Dammermann A, Pemble H, Monen J, Portier N, Hyman A, Desai A, Oegema K. (2008) Expression and imaging of fluorescent proteins in the C. elegans gonad and early embryo. Methods Cell Biol. 85:179-218
Cheeseman IM, Desai A. (2008) Molecular architecture of the kinetochore-microtubule interface. Nat Rev Mol Cell Biol. 9(1):33-46
Cheeseman IM, Hori T, Fukagawa T, Desai A. (2008) KNL1 and the CENP-H/I/K complex coordinately direct kinetochore assembly in vertebrates. Mol Biol Cell 19(2):587-594
2007
Liu J, Desai A, Onuchic JN, Hwa T. (2007) A mechanobiochemical mechanism for monooriented chromosome oscillation in mitosis. Proc Natl Acad Sci U S A. 104(41):16104-16109
Audhya A, McLeod IX, Yates JR, Oegema K. (2007) MVB-12, a fourth subunit of metazoan ESCRT-I, functions in receptor downregulation. PLoS One. 2(9):e956
Sandall S, Desai A. (2007) When it comes to couple(r)s, do opposites attract? at Struct Mol Biol. 14(9):790-792
Audhya A, Desai A, Oegema K. (2007) A role for Rab5 in structuring the endoplasmic reticulum. J Cell Biol. 178(1):43-56
Maddox AS, Lewellyn L, Desai A, Oegema K. (2007) Anillin and the septins promote asymmetric ingression of the cytokinetic furrow. Dev Cell. 12(5):827-835
Portier N, Audhya A*, Maddox PS*, Green RA, Dammermann A, Desai A, Oegema K. (2007) A microtubule-independent role for centrosomes and aurora a in nuclear envelope breakdown. Dev Cell. 12(4):515-529
Maddox PS, Hyndman F, Monen J, Oegema K, Desai A. (2007) Functional genomics identifies a Myb domain-containing protein family required for assembly of CENP-A chromatin. J Cell Biol. 176(6):757-763
Black BE, Jansen LE, Maddox PS, Foltz DR, Desai AB, Shah JV, Cleveland DW. (2007) Centromere identity maintained by nucleosomes assembled with histone H3 containing the CENP-A targeting domain. Mol Cell. 25(2):309-322
Schlaitz AL, Srayko M, Dammermann A, Quintin S, Wielsch N, MacLeod I, de Robillard Q, Zinke A, Yates JR 3rd, Müller-Reichert T, Shevchenko A, Oegema K, Hyman AA. (2007) The C. elegans RSA complex localizes protein phosphatase 2A to centrosomes and regulates mitotic spindle assembly. Cell. 128(1):115-127
Gassmann R, Kline SL, Carvalho A, Desai A. (2007) Analysis of kinetochore assembly and function in Caenorhabditis elegans embryos and human cells. Methods. 41:177-189
2006
McNally K, Audhya A, Oegema K, McNally FJ. (2006) Katanin controls mitotic and meiotic spindle length. J Cell Biol. 175(6):881-891
Sandall S, Severin F, McLeod IX, Yates JR 3rd, Oegema K, Hyman A, Desai A. (2006) A Bir1-Sli15 complex connects centromeres to microtubules and is required to sense kinetochore tension. Cell. 127(6):1179-1191
Smolka MB, Chen SH, Maddox PS, Enserink JM, Albuquerque CP, Wei XX, Desai A, Kolodner RD, Zhou H. (2006) An FHA domain-mediated protein interaction network of Rad53 reveals its role in polarized cell growth. J Cell Biol. 175(5):743-753
Cheeseman IM, Chappie JS, Wilson-Kubalek EM, Desai A. (2006) The conserved KMN network constitutes the core microtubule-binding site of the kinetochore. Cell. 127(5):983-997
Maddox PS, Portier N, Desai A, Oegema K. (2006) Molecular analysis of mitotic chromosome condensation using a quantitative time-resolved fluorescence microscopy assay. Proc Natl Acad Sci U S A. 103(41):15097-15102
Olson SK, Bishop JR, Yates JR, Oegema K, Esko JD. (2006) Identification of novel chondroitin proteoglycans in Caenorhabditis elegans: embryonic cell division depends on CPG-1 and CPG-2. J Cell Biol. 173(6):985-994
Sato K, Sato M, Audhya A, Oegema K, Schweinsberg P, Grant BD. (2006) Dynamic regulation of caveolin-1 trafficking in the germ line and embryo of Caenorhabditis elegans. Mol Biol Cell. 17(7):3085-3094
Okada M, Cheeseman IM, Hori T, Okawa K, McLeod IX, Yates JR 3rd, Desai A, Fukagawa T. (2006) The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. Nat Cell Biol. 8(5):446-457
Kline SL, Cheeseman IM*, Hori T, Fukagawa T, Desai A. (2006) The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation. J Cell Biol. 173(1):9-17
Budde PP, Desai A, Heald R. (2006) Analysis of microtubule polymerization in vitro and during the cell cycle in Xenopus egg extracts. Methods. 38:29-34
Oegema K, Hyman AA. (2006) Cell division. WormBook 19:1-40
2005
Maddox AS, Habermann B, Desai A, Oegema K. (2005) Distinct roles for two C. elegans anillins in the gonad and early embryo. Development. 132(12):2837-2848
Mitchison TJ, Maddox P, Gaetz J, Groen A, Shirasu M, Desai A, Salmon ED, Kapoor TM. (2005) Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindles. Mol Biol Cell. 16(6):3064-3076
Monen J, Maddox PS, Hyndman F, Oegema K, Desai A. (2005) Differential role of CENP-A in the segregation of holocentric C. elegans chromosomes during meiosis and mitosis. Nat Cell Biol. 7(12):1248-1255
Bomont P, Maddox P, Shah JV, Desai AB, Cleveland DW. (2005) Unstable microtubule capture at kinetochores depleted of the centromere-associated protein CENP-F. EMBO J. 24:3927-3939
Audhya A, Hyndman F, McLeod IX, Maddox AS, Yates JR 3rd, Desai A, Oegema K. (2005) A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans. J Cell Biol. 171(2):267-279
Mao Y, Desai A, Cleveland DW. (2005) Microtubule capture by CENP-E silences BubR1-dependent mitotic checkpoint signaling. J Cell Biol. 170:873-880
Ozlu N, Srayko M, Kinoshita K, Habermann B, O'Toole E T, Muller-Reichert T, Schmalz N, Desai A, Hyman AA. (2005) An essential function of the C. elegans ortholog of TPX2 is to localize activated aurora A kinase to mitotic spindles. Dev Cell. 9:237-248
Maddox AS, Habermann B, Desai A, Oegema K. (2005) Distinct roles for two C. elegans anillins in the gonad and early embryo. Development. 132(12):2837-2848
Cheeseman IM, MacLeod I, Yates JR 3rd, Oegema K, Desai A. (2005) The CENP-F-like proteins HCP-1 and HCP-2 target CLASP to kinetochores to mediate chromosome segregation. Curr Biol. 15(8):771-777
Sonnichsen B, Koski LB, Walsh A, Marschall P, Neumann B, Brehm M, Alleaume AM, Artelt J, Bettencourt P, Cassin E, Hewitson M, Holz C, Khan M, Lazik S, Martin C, Nitzsche B, Ruer M, Stamford J, Winzi M, Heinkel R, Roder M, Finell J, Hantsch H, Jones SJ, Jones M, Piano F, Gunsalus KC, Oegema K, Gonczy P, Coulson A, Hyman AA, Echeverri CJ. (2005) Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans. Nature. 434(7032):462-469
Wittmann T, Desai A. (2005) Microtubule cytoskeleton: a new twist at the end. Curr Biol. 15(4):R126-129
Kline-Smith SL, Sandall S, Desai A. (2005) Kinetochore-spindle microtubule interactions during mitosis. Curr Opin Cell Biol. 17(1):35-46
Cheeseman IM, Desai A. (2005) A combined approach for the localization and tandem affinity purification of protein complexes from metazoans. Sci STKE 2005(266):pl1
2004
Dammermann A, Müller-Reichert T, Pelletier L, Habermann B, Desai A, Oegema K. (2004) Centriole assembly requires both centriolar and pericentriolar material proteins. Dev Cell. 7(6):815-829
Mitchison TJ, Maddox P, Groen A, Cameron L, Perlman Z, Ohi R, Desai A, Salmon ED, Kapoor TM. (2004) Bipolarization and poleward flux correlate during Xenopus extract spindle assembly. Mol Biol Cell. 15(12):5603-5615
Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR 3rd, Oegema K, Desai A. (2004) A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension. Genes Dev. 18(18):2255-2268
Maddox PS, Oegema K, Desai A, Cheeseman IM. (2004) "Holo"er than thou: chromosome segregation and kinetochore function in C. elegans. Chromosome Res. 12(6):641-653
Cheeseman IM, Desai A. (2004) Cell division: feeling tense enough? Nature. 428(6978):32-33
Cheeseman IM, Desai A. (2004) Cell division: AAAtacking the mitotic spindle. Curr Biol. 14(2):R70-72
2003
Desai A, Rybina S, Müller-Reichert T, Shevchenko A, Shevchenko A, Hyman A, Oegema K. (2003) KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans. Genes Dev. 17(19):2421-2435
Maddox AS, Oegema K. (2003) Deconstructing cytokinesis. Nat Cell Biol. 5(9):773-776
Dammermann A, Desai A, Oegema K. (2003) The minus end in sight. Curr Biol. 13(15):R614-624
Maddox AS, Oegema K. (2003) Closing the GAP: a role for a RhoA GAP in cytokinesis. Mol Cell. 11(4):846-848
Kirkham M, Muller-Reichert T, Oegema K, Grill S, Hyman AA. (2003) SAS-4 is a C. elegans centriolar protein that controls centrosome size. Cell. 112(4):575-587
Moritz, M., Zheng, Y., Alberts, B.M., Oegema, K. (2003) Recruitment of the γ-Tubulin Ring Complex to Drosophila Salt-stripped Centrosome Scaffolds. The Journal of Cell Biology. 142(3): 775-786.
2002
Walczak CE, Gan EC, Desai A, Mitchison TJ, Kline-Smith SL. (2002) The microtubule-destabilizing kinesin XKCM1 is required for chromosome positioning during spindle assembly. Curr Biol. 12(21):1885-1889
Desai A. (2002) The (theoretical) yin and yang of spindle mechanics. Dev Cell. 3(4):465-467
Maddox P, Desai A, Oegema K, Mitchison TJ, Salmon ED. (2002) Poleward microtubule flux is a major component of spindle dynamics and anaphase a in mitotic Drosophila embryos. Curr Biol. 12(19):1670-1674
Hannak E, Oegema K, Kirkham M, Gonczy P, Habermann B, Hyman AA. (2002) The kinetically dominant assembly pathway for centrosomal asters in Caenorhabditis elegans is gamma-tubulin dependent. J Cell Biol. 157(4):591-602
2001
Hannak E, Kirkham M, Hyman AA, Oegema K. (2001) Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans. J Cell Biol. 155(7):1109-1116
Cheeseman IM, Brew C, Wolyniak M, Desai A, Anderson S, Muster N, Yates JR, Huffaker TC, Drubin DG, Barnes G. (2001) Implication of a novel multiprotein Dam1p complex in outer kinetochore function. J Cell Biol. 155(7):1137-1145
Oegema K, Desai A, Rybina S, Kirkham M, Hyman AA. (2001) Functional analysis of kinetochore assembly in Caenorhabditis elegans. J Cell Biol. 153(6):1209-1226
Wittmann, T., Hyman, A., Desai, A. (2001). The spindle: a dynamic assembly of microtubules and motors. Nat Cell Biol. 3(1): E28-E34.